A detailed protocol for expression, purification, and activity determination of recombinant SaCas9 - ScienceDirect
MPs | Free Full-Text | A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
Purification of Cas9 protein. (A) SDS-PAGE (sodium dodecyl sulfate... | Download Scientific Diagram
Improving the efficiency of CRISPR-Cas9: The expression & purification of Cas9
Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2 | Nature Communications
Purifying Cas Proteins and Complexes: Part I- TriAltus Bioscience Blog
MPs | Free Full-Text | A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
Structural insights into the human PA28–20S proteasome enabled by efficient tagging and purification of endogenous proteins | PNAS
Cas9 Proteins • NUCLEUS BIOTECH
Addgene: CRISPR Plasmids - Purify Genomic Loci
A detailed protocol for expression, purification, and activity determination of recombinant SaCas9 - ScienceDirect
CRISPR-Cas9/Cas12A expression, purification and validation - Genome Innovation Hub - University of Queensland
CRISPR-Cas9 Protein – TriAltus Bioscience
CRISPR-Cas9-induced gene knockout in zebrafish
Direct purification of CRISPR/Cas ribonucleoprotein from E. coli in a single step
Establishment of an efficient genotyping technique based on targeted DNA endonuclease <i>in vitro</i> activity of CRISPR/Cas9 ribonucleoprotein
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates | Science Advances
Cas9 Nuclease
Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins | Scientific Reports
A Novel Set of Cas9 Fusion Proteins to stimulate Homologous Recombination: Cas9-HRs | bioRxiv
STANDARD OPERATING PROCEDURE DETAILS
Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins | Communications Biology
Introduction to the CRISPR/Cas9 system
Isolating Genomic Regions of Interest with the CRISPR System
Undergraduate Honors Thesis | Improving the efficiency of CRISPR-Cas9: The expression & purification of Cas9 | ID: g732d951z | CU Scholar
The Cas9-gRNA ribonucleoprotein complex-mediated editing of pyrG in Ganoderma lucidum and unexpected insertion of contaminated DNA fragments | Scientific Reports
Improving the efficiency of CRISPR-Cas9: The expression & purification of Cas9
Purification of Recombinant Cas9 – openlabnotebooks.org
